Salmon Sperm DNA

Catalog No. ABIN925593

Supplier Product No.: mb-103-0025

Quick Overview for Salmon Sperm DNA (ABIN925593)

Product Details

Supplier Product No.: mb-103-0025

Supplier: Rockland

Characteristics: Concentration Definition: by UV absorbance at 260 nm

Purity: A260/A280: 1.85 % Hyperchromocity: 30.5

Application Details

Application Notes: This product is a convenient, ready-to-use solution of salmon sperm DNA especially prepared for use in the preparation of pre-hybridization and hybridization solutions and as a DNA carrier in yeast transformation protocols and other related methods. This solution contains sheared single-stranded DNA molecules that can be used to block the non-specific attachment of probe DNA to the surface of a membrane (Southern) or to increase yeast transformation efficiency.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 5.0 mg/mL

Buffer: 0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6

Storage: -20 °C

Target Details

Background: Prepared by a modification of the method of Emanuel and Chaikoff, JBC 203, 164 (1953) from salmon testes DNA by mechanical shearing and heat denaturation to an average size of 100 to 200 base pairs. To reverse any renaturation occurring during storage, this material should be briefly boiled and rapidly chilled prior to use. >75% native nucleic acid.